Homogenization Buffer for Drosophila
| Reagent | Distributor and catalog number | Composition |
|---|---|---|
| Liberase (13 Wunsch U/mL) | Roche 05401119001 | 40 µL (0.26 Wunsch U/mL) |
| Elastase (20 mg/mL) | Promega V189A | 8 µL (80 µg/mL final) |
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Add to DPBS (Thermo Fisher 14190094) to a final volume of 2 mL. Freshly prepare and use within 15 min of preparation. Sterilization by filtration or other methods should be avoided to prevent loss of activity.
