Paraformaldehyde (4%, w/v) in PBS
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1. In a fume hood, weigh and dissolve paraformaldehyde powder (Nacalai Tesque 26126-54) to prepare a 4% (w/v) formaldehyde solution in Phosphate-buffered saline (PBS). Prepare slightly less than the final required volume.
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2. Heat the solution to 60°C on a hot plate using a magnetic stirrer until completely dissolved.
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3. Adjust the pH of this solution to 7.4 using dilute (1 m) HCl. Top up the solution to the final volume after pH adjustment with PBS.
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For experimental ease, it is possible to use stored frozen aliquots. However, using fresh 4% PFA is optimal for the best results.
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