Recipe

TNTB

Reagent stock Volume required for 50 mL Final concentration
Tris HCl (Roche 10812846001), 1 m,
 pH 9.5 		5 mL 100 mm
NaCl, 5 m 1.5 mL 150 mm
Tween-20 (VWR 97062-332),
 10% (v/v) stock 		250 µL 0.05%
Blocking Reagent in MAB 5 mL 1%
H2O Adjust to 50 mL

  • Freshly prepare for each experiment.

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  1. doi:10.1101/pdb.rec108310 Cold Spring Harb Protoc 2024: pdb.rec108310- © 2024 Cold Spring Harbor Laboratory Press
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