Wash Solution for Mosquito Tissues
| Reagent | Amount | Final concentration |
|---|---|---|
| 10× Phosphate-buffered saline without calcium or magnesium (Lonza 17-517Q) | 5 mL | 1× |
| 10% Triton X-100 (Sigma-Aldrich 93443 diluted 1/10 in H2O) | 1.25 mL | 0.25% |
| Double-distilled H2O | 43.75 mL |
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Makes 50 mL. Store for up to 6 mo at room temperature.
