Antibody Buffer
| Reagent | Amount | Final concentration |
|---|---|---|
| 10× Phosphate-buffered saline without calcium or magnesium (Lonza 17-517Q) | 1 mL | 1× |
| 10% Triton X-100 (Sigma-Aldrich 93443 diluted 1/10 in H2O) | 0.25 mL | 0.25% |
| Normal goat serum (Jackson ImmunoResearch 005-000-121 rehydrated in 10 mL double-distilled H2O) | 0.2 mL | 2% |
| Double-distilled H2O | 8.55 mL |
-
Makes 10 mL. Split into 1-mL aliquots in 1.5-mL tubes on ice, and store for up to 6 mo at −20°C.
