Recipe

TE Buffer (10×, pH 8.0)

To make 100 mL of solution, mix 10 mL of 1 m Tris-HCl (pH 8.0) and 2 mL of 0.5 m EDTA (pH 8.0; Invitrogen 15575020) with 80 mL of double-distilled H2O. Bring the final volume to 100 mL with double-distilled H2O. Autoclave to sterilize.

Store for up to 1 yr at room temperature.

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  1. doi:10.1101/pdb.rec108543 Cold Spring Harb Protoc 2025: pdb.rec108543- © 2024 Cold Spring Harbor Laboratory Press

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