Recipe

Wash Buffer III for ChIP

Reagent Stock solution Manufacturer information Volume Final concentration
LiCl 5 m Fisher Scientific L120 500 μL 250 mm
Igepal CA-630 10% (v/v) Sigma-Aldrich I8896 500 μL 0.5% (v/v)
Na-deoxycholate 10% (w/v) bioPLUS 40430018 500 μL 0.5% (w/v)
TE buffer, 10× 10×, pH 8.0 1 mL 1×
Nuclease-free H2O 7500 μL

  • Makes a total of 10 mL. Freshly prepare.

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  1. doi:10.1101/pdb.rec108544 Cold Spring Harb Protoc 2025: pdb.rec108544- © 2024 Cold Spring Harbor Laboratory Press
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