General screening funnel for antibody lead identification. During a phage display-based antibody discovery campaign, polyclonal pools of binders to the target are enriched during phage panning, and then single antibody binders are identified in the primary screen. These then move to secondary screening, with select hits moving into in vivo testing and biophysical optimization and engineering. At each step, the number of antibodies is triaged, starting with hundreds or thousands of antibodies down to a few.