Recipe

HCR Amplification Buffer

Use 50% (w/v) dextran sulfate (EMD Millipore S4030), sodium chloride-sodium citrate buffer (SSC; 20×), and Tween 20 (Fisher J20605-AP) to prepare a 5% (w/v) dextran sulfate, 5× SSC, and 0.1% (v/v) Tween 20 solution in pure water. Store at room temperature for up to 6 mo.

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  1. doi:10.1101/pdb.rec108592 Cold Spring Harb Protoc 2025: pdb.rec108592- © 2025 Cold Spring Harbor Laboratory Press

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