Probe Hybridization Buffer
30% (w/v) formamide
5× SSC (dilute from sodium chloride-sodium citrate buffer [SSC; 20×])
9 mm citric acid, pH 6
0.1% (v/v) Tween 20 (Fisher J20605-AP)
50 µg/mL heparin (Fisher BP2425)
1× Denhardt's solution (Alfa Aesar J63135)
5% (w/v) dextran sulfate (EMD Millipore S4030)
Combine reagents in DEPC-treated water and store for up to 6 mo at −20°C.
