Schematic diagram of the planned chimeric antigen receptor (CAR)-coding sequence. This sequence encodes a rabbit scFv that specifically binds an antigen on the surface of Carlos's tumor cells. It is connected through a CD8 hinge and transmembrane (TM) to the intracellular domains of coreceptor protein CD3 ζ and costimulatory protein 4-1BB. The functions of the intracellular domains are explained in the text. CAR T cells (T cells expressing the CAR gene) deploy the CAR on the cell surface, with the single-chain variable fragment (scFv) exposed extracellularly, and the 4-1BB and CD3 ζ domains exposed intracellularly. Data are from Bhoj et al. (2021).
Figure 9.

Schematic diagram of the planned chimeric antigen receptor (CAR)-coding sequence. This sequence encodes a rabbit scFv that specifically binds an antigen on the surface of Carlos's tumor cells. It is connected through a CD8 hinge and transmembrane (TM) to the intracellular domains of coreceptor protein CD3 ζ and costimulatory protein 4-1BB. The functions of the intracellular domains are explained in the text. CAR T cells (T cells expressing the CAR gene) deploy the CAR on the cell surface, with the single-chain variable fragment (scFv) exposed extracellularly, and the 4-1BB and CD3 ζ domains exposed intracellularly. Data are from Bhoj et al. (2021).

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  1. Published in Advance October 22, 2025, doi: 10.1101/pdb.over107753 Cold Spring Harb Protoc 2026: pdb.over107753-
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