Recipe

Sodium Acetate (3 m, pH 5.2)

Dissolve 246.1 g of sodium acetate in 500 mL of deionized H2O. Adjust the pH to 5.2 with glacial acetic acid. Allow the solution to cool overnight. Adjust the pH once more to 5.2 with glacial acetic acid. Adjust the final volume to 1 L with deionized H2O, and filter-sterilize with a 0.5-μm filter into an autoclaved bottle. Store for up to 2 mo at room temperature.

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  1. doi:10.1101/pdb.rec108664 Cold Spring Harb Protoc 2026: pdb.rec108664- © 2026 Cold Spring Harbor Laboratory Press

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