SOC Medium
| Reagent | Final concentration |
|---|---|
| Tryptone | 2% (w/v) |
| Yeast extract | 0.5% (w/v) |
| NaCl | 10 mm |
| KCl | 2.5 mm |
Add these ingredients to water in a flask and autoclave at 121°C. Allow the solution to cool. Add sterile MgCl2 to a final concentration of 10 mm and sterile glucose to a final concentration of 20 mm. Store at room temperature.
