Kanamycin Stock Solution
Weigh 0.5 g of kanamycin (GoldBio K-120 [CAS25389-94-0, MW = 582.6]). Dissolve into 10 mL of double-distilled sterile H2O (sterile ddH2O). Prewet a 0.22-μm syringe filter by drawing through 5–10 mL of sterile ddH2O, and then discard the water. Sterilize the kanamycin stock by passage through the prepared 0.22-μm syringe filter. Make 1-mL aliquots in sterile microcentrifuge tubes. Stock may be kept for 1 yr at −20°C.
