Table of Contents

Protocols

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  Preparation of Reversed-Phase Microcolumns

  • Martin R. Larsen

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4606
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  Phosphopeptide Purification by IMAC with Fe(III) and Ga(III)

  • Hanno Steen,
  • Allan Stensballe,
  • and Ole N. Jensen

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4607
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  Preparation and Use of Microcolumns for Sample Desalting or Nanoscale IMAC

  • Hanno Steen,
  • Allan Stensballe,
  • and Ole N. Jensen

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4608
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  Preparation of Immobilized Enzyme Microcolumns

  • Martin R. Larsen

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4609
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  Offline Micro-IMAC Enrichment of Phosphoproteins

  • Garry L. Corthals,
  • Valerie C. Wasinger,
  • and David R. Goodlett

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4624
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  Analysis of Phosphopeptides by μLC-ESI-MS/MS

  • Garry L. Corthals,
  • Valerie C. Wasinger,
  • and David R. Goodlett

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4625
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  Imaging Mouse Embryos after Whole-Mount In Situ Hybridization

  • Maki Wakamiya

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4774
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  Sectioning Mouse Embryos after Whole-Mount In Situ Hybridization

  • Michael Shen

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4775
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  Handling Mouse Blastocysts for Fixation

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4814
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  Embedding Mouse Embryos and Tissues in Wax

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4815
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  Preparing Glass Slides and Coverslips for In Situ Hybridization

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4817
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  Dewaxing and Rehydrating Sections prior to In Situ Hybridization

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4818
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  In Situ Hybridization of Mouse Embryo and Tissue Sections with Radiolabeled RNA Probes

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4821
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  In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Preparation of Embryos and Probes

  • Thomas Lufkin

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4822
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  In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry

  • Thomas Lufkin

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4823
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  Imaging Real-Time Gene Expression in Mammalian Cells with Single-Transcript Resolution

  • Amber L. Wells,
  • John S. Condeelis,
  • Robert H. Singer,
  • and Daniel Zenklusen

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4869
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  Imaging Real-Time Gene Expression in Living Yeast

  • Daniel Zenklusen,
  • Amber L Wells,
  • John S. Condeelis,
  • and Robert H. Singer

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4870
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  Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Image Analysis of Single mRNA Transcripts

  • Amber L. Wells,
  • John S. Condeelis,
  • Robert H. Singer,
  • and Daniel Zenklusen

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4871
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  Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Single mRNA Particle Tracking with ImageJ-Based Analysis

  • Amber L. Wells,
  • John S. Condeelis,
  • Robert H. Singer,
  • and Daniel Zenklusen

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4872
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  Micrococcal Nuclease-Southern Blot Assay: I. MNase and Restriction Digestions

  • Michael Carey and
  • Stephen T. Smale

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4890
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  Micrococcal Nuclease-Southern Blot Assay: II. Capillary Transfer and Hybridization

  • Michael Carey and
  • Stephen T. Smale

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4891
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  Fate-Mapping Technique: Grafting Fluorescent Cells into Gastrula-Stage Mouse Embryos at 7-7.5 Days Post-coitum

  • Vanessa J. Franklin,
  • Heidi Bildsoe,
  • and Patrick P.L. Tam

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4892
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  Fate-Mapping Technique: Targeted Whole-Embryo Electroporation of DNA Constructs into the Germ Layers of Mouse Embryos 7-7.5 Days Post-coitum

  • P.-L. Khoo,
  • V.J. Franklin,
  • and P.P.L. Tam

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4893
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  Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content

  • Ingrid Schmid

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4895
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