Table of Contents

December 2008; 2008 (12)

Emerging Model Organisms

  • The Demosponge Amphimedon queenslandica: Reconstructing the Ancestral Metazoan Genome and Deciphering the Origin of Animal Multicellularity

    • Bernard M. Degnan,
    • Maja Adamska,
    • Alina Craigie,
    • Sandie M. Degnan,
    • Bryony Fahey,
    • Marie Gauthier,
    • John N.A. Hooper,
    • Claire Larroux,
    • Sally P. Leys,
    • Erica Lovas,
    • and Gemma S. Richards
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.emo108

  • Dictyostelium discoideum: The Social Ameba

    • Pascale Gaudet,
    • Petra Fey,
    • and Rex Chisholm
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.emo109

  • The Two-Spotted Cricket Gryllus bimaculatus: An Emerging Model for Developmental and Regeneration Studies

    • Taro Mito and
    • Sumihare Noji
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.emo110

  • The Dogfish Scyliorhinus canicula: A Reference in Jawed Vertebrates

    • Marion Coolen,
    • Arnaud Menuet,
    • Danièle Chassoux,
    • Claudia Compagnucci,
    • Sébastien Henry,
    • Laurent Lévèque,
    • Corinne Da Silva,
    • Frédérick Gavory,
    • Sylvie Samain,
    • Patrick Wincker,
    • Claude Thermes,
    • Yves D’Aubenton-Carafa,
    • Isabel Rodriguez-Moldes,
    • Gavin Naylor,
    • Michael Depew,
    • Pascal Sourdaine,
    • and Sylvie Mazan
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.emo111

Information Panels

  • Using the Nucleic Acid Programmable Protein Array (NAPPA) for Identifying Protein-Protein Interactions: General Guidelines

    • Andrew J. Link and
    • Joshua LaBaer
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.ip62

Protocols

  • Bone Marrow-Derived Macrophages (BMM): Isolation and Applications

    • Joachim Weischenfeldt and
    • Bo Porse
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5080

  • Gene Silencing by Injection and Electroporation of dsRNA in Avian Embryos

    • Olivier Mauti,
    • Thomas Baeriswyl,
    • and Esther T. Stoeckli
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5094

  • Isolation of Amphimedon Developmental Material

    • Sally P. Leys,
    • Claire Larroux,
    • Marie Gauthier,
    • Maja Adamska,
    • Bryony Fahey,
    • Gemma S. Richards,
    • Sandie M. Degnan,
    • and Bernard M. Degnan
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5095

  • Whole-Mount In Situ Hybridization in Amphimedon

    • Claire Larroux,
    • Bryony Fahey,
    • Maja Adamska,
    • Gemma S. Richards,
    • Marie Gauthier,
    • Kathryn Green,
    • Erica Lovas,
    • and Bernard M. Degnan
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5096

  • Analysis of Cell Movement in Amphimedon Embryos by Injection of Fluorescent Tracers

    • Maja Adamska and
    • Bernard M. Degnan
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5097

  • Genotyping Individual Amphimedon Embryos, Larvae, and Adults

    • Sandie M. Degnan,
    • Alina Craigie,
    • and Bernard M. Degnan
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5098

  • Growth and Maintenance of Dictyostelium Cells

    • Pascale Gaudet,
    • Petra Fey,
    • and Rex Chisholm
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5099

  • Multicellular Development of Dictyostelium

    • Pascale Gaudet,
    • Petra Fey,
    • and Rex Chisholm
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5100

  • Making Permanent Stocks of Dictyostelium

    • Pascale Gaudet,
    • Petra Fey,
    • and Rex Chisholm
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5101

  • Transformation of Dictyostelium with Plasmid DNA by Calcium Phosphate Precipitation

    • Pascale Gaudet,
    • Petra Fey,
    • and Rex Chisholm
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5102

  • Transformation of Dictyostelium with Plasmid DNA by Electroporation

    • Pascale Gaudet,
    • Petra Fey,
    • and Rex Chisholm
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5103

  • Selection of Dictyostelium Transformants

    • Pascale Gaudet,
    • Petra Fey,
    • and Rex Chisholm
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5104

  • Extraction of DNA from Dictyostelium

    • Pascale Gaudet,
    • Petra Fey,
    • and Rex Chisholm
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5105

  • Extraction of RNA from Dictyostelium

    • Pascale Gaudet,
    • Petra Fey,
    • and Rex Chisholm
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5106

  • Cultures of Cerebellar Granule Neurons

    • Parizad M. Bilimoria and
    • Azad Bonni
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5107

  • Using the Nucleic Acid Programmable Protein Array (NAPPA) for Identifying Protein-Protein Interactions. Protocol 1: Coexpression of Query Protein on NAPPA Slides

    • Andrew J. Link and
    • Joshua LaBaer
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5108

  • Using the Nucleic Acid Programmable Protein Array (NAPPA) for Identifying Protein-Protein Interactions. Protocol 2: Detection of Query Proteins on NAPPA Slides

    • Andrew J. Link and
    • Joshua LaBaer
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5109

  • Collection, Cryopreservation, Storage, and Revitalization of Transgenic Mouse Embryos

    • Anna Schwab and
    • Johannes Schenkel
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5111

  • Chicken Erythrocyte Histone Octamer Preparation

    • Craig L. Peterson and
    • Jeffrey C. Hansen
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5112

  • Salt Gradient Dialysis Reconstitution of Nucleosomes

    • Craig L. Peterson
    Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5113
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December 2008; 2008 (12)
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