Table of Contents

Protocols

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  Inoculation and Passaging of Mammalian Monolayer Cell Cultures

  • Gretchen J. Darlington

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4347
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  Quantifying Protein by Bicinchoninic Acid

  • Richard J. Simpson

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4722
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  The PAS Reaction for Staining Cell Walls

  • Stuart Baum

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4956
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  Phenotypic Analysis of Arabidopsis Mutants: Trypan Blue Stain for Fungi, Oomycetes, and Dead Plant Cells

  • Saskia van Wees

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4982
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  Cryosectioning Tissues

  • Andrew H. Fischer,
  • Kenneth A. Jacobson,
  • Jack Rose,
  • and Rolf Zeller

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4991
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  Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version A

  • Gustavo Tiscornia,
  • Oded Singer,
  • and Inder M. Verma

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5009
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  Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version B

  • Gustavo Tiscornia,
  • Oded Singer,
  • and Inder M. Verma

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5010
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  Phage-Display Methodology for the Study of Protein-Protein Interactions: Stage 1, Generating Libraries

  • Carl S. Goodyear and
  • Gregg J. Silverman

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5020
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  Phage-Display Methodology for the Study of Protein-Protein Interactions: Stage 2, Panning

  • Carl S. Goodyear and
  • Gregg J. Silverman

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5021
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  Phage-Display Methodology for the Study of Protein-Protein Interactions: Stage 3, Binding Analysis

  • Carl S. Goodyear and
  • Gregg J. Silverman

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5022
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  Analyzing DNA Replication I: Labeling Animals, Tissues, and Cells with Bromodeoxyuridine (BrdU)

  • Dean Jackson and
  • Peter R. Cook

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5031
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  Analyzing DNA Replication II: Fixation and Processing of Tissues and Cells Labeled with Bromodeoxyuridine (BrdU)

  • Dean Jackson and
  • Peter R. Cook

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5032
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  Analyzing DNA Replication III: Antibody Labeling of Incorporated Bromodeoxyuridine (BrdU) in Tissues and Cells

  • Dean Jackson and
  • Peter R. Cook

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5033
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  In Vitro Assays for the Extracellular Matrix Protein-Regulated Extravasation Process

  • Chaoyu Ma and
  • Xiao-Fan Wang

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5034
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  Whole Mount RNA In Situ Hybridization on Zebrafish Embryos: Probe Synthesis

  • Cecilia Moens

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5036
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  Whole Mount RNA In Situ Hybridization on Zebrafish Embryos: Hybridization

  • Cecilia Moens

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5037
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  Whole Mount RNA In Situ Hybridization on Zebrafish Embryos: Mounting

  • Cecilia Moens

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5038
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  Isolation of Centrosomes from Cultured Mammalian Cells

  • Thomas E. Meigs and
  • Daniel D. Kaplan

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5039
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