Table of Contents

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Construction and Characterization of Adenovirus Vectors

P. Joel Ross and
Robin J. Parks

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5011

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Microinjection of Bichir (Polypterus) Embryos

Masaki Takeuchi,
Masataka Okabe,
and Shinichi Aizawa

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5157

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Whole-Mount In Situ Hybridization of Bichir (Polypterus) Embryos

Masaki Takeuchi,
Masataka Okabe,
and Shinichi Aizawa

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5158

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Computational Docking of Biomolecular Complexes with AutoDock

David S. Goodsell

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5200

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Culture and Propagation of Laboratory Populations of the African Butterfly Bicyclus anynana

Paul M. Brakefield,
Patrícia Beldade,
and Bas J. Zwaan

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5203

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Surgical Manipulations on Pupal Wings from the African Butterfly Bicyclus anynana: Damage and Cauteries

Paul M. Brakefield,
Patrícia Beldade,
and Bas J. Zwaan

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5204

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Surgical Manipulations on Pupal Wings from the African Butterfly Bicyclus anynana: Grafts

Paul M. Brakefield,
Patrícia Beldade,
and Bas J. Zwaan

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5205

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Fixation and Dissection of Embryos from the African Butterfly Bicyclus anynana

Paul M. Brakefield,
Patrícia Beldade,
and Bas J. Zwaan

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5206

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Dissection of Larval and Pupal Wings from the African Butterfly Bicyclus anynana

Paul M. Brakefield,
Patrícia Beldade,
and Bas J. Zwaan

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5207

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In Situ Hybridization of Embryos and Larval and Pupal Wings from the African Butterfly Bicyclus anynana

Paul M. Brakefield,
Patrícia Beldade,
and Bas J. Zwaan

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5208

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Immunohistochemistry Staining of Embryos from the African Butterfly Bicyclus anynana

Paul M. Brakefield,
Patrícia Beldade,
and Bas J. Zwaan

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5209

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Immunohistochemistry Staining of Wing Discs from the African Butterfly Bicyclus anynana

Paul M. Brakefield,
Patrícia Beldade,
and Bas J. Zwaan

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5210

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Extraction and Gas Chromatography Analysis of Adult Pheromones from the African Butterfly Bicyclus anynana

Paul M. Brakefield,
Patrícia Beldade,
and Bas J. Zwaan

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5211

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Fresh Weight, Dry Weight, and Fat Content of Adult African Butterflies Bicyclus anynana

Paul M. Brakefield,
Patrícia Beldade,
and Bas J. Zwaan

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5212

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Constant Volume Respirometry in the African Butterfly Bicyclus anynana

Paul M. Brakefield,
Patrícia Beldade,
and Bas J. Zwaan

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5213

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Hemolymph Extraction from Various Developmental Stages of the African Butterfly Bicyclus anynana

Paul M. Brakefield,
Patrícia Beldade,
and Bas J. Zwaan

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5214

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Injection of Chemicals into Pupae of the African Butterfly Bicyclus anynana

Paul M. Brakefield,
Patrícia Beldade,
and Bas J. Zwaan

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5215

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Preparation and Immunolabeling of Caenorhabditis elegans

Sarah Crittenden and
Judith Kimble

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5216

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Construction of Adenovirus Vectors with RGD-Modified Fiber for Transductional Targeting

Yosuke Kawakami and
David T. Curiel

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5217

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Construction of Fusion Proteins for Transductional Targeting

Yosuke Kawakami and
David T. Curiel

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5218

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Construction of Adenovirus Vectors for Transcriptional Targeting

Yosuke Kawakami and
David T. Curiel

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5219

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Comet Fluorescence In Situ Hybridization (Comet-FISH): Detection of DNA Damage

Wiebke Schlörmann and
Michael Glei

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5220

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Systematic Monitoring of Protein Complex Composition and Abundance by Blue-Native PAGE

Holger Eubel and
A. Harvey Millar

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5221

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Metabolic Footprinting for the Study of Microbial Biodiversity

Georgina A. Pope,
Donald A. MacKenzie,
Marianne Defernez,
and Ian N. Roberts

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5222

Emerging Model Organisms

The Genus Polypterus (Bichirs): A Fish Group Diverged at the Stem of Ray-Finned Fishes (Actinopterygii)

The African Butterfly Bicyclus anynana: A Model for Evolutionary Genetics and Evolutionary Developmental Biology

Protocols

Construction and Characterization of Adenovirus Vectors

Microinjection of Bichir (Polypterus) Embryos

Whole-Mount In Situ Hybridization of Bichir (Polypterus) Embryos

Computational Docking of Biomolecular Complexes with AutoDock

Culture and Propagation of Laboratory Populations of the African Butterfly Bicyclus anynana

Surgical Manipulations on Pupal Wings from the African Butterfly Bicyclus anynana: Damage and Cauteries

Surgical Manipulations on Pupal Wings from the African Butterfly Bicyclus anynana: Grafts

Fixation and Dissection of Embryos from the African Butterfly Bicyclus anynana

Dissection of Larval and Pupal Wings from the African Butterfly Bicyclus anynana

In Situ Hybridization of Embryos and Larval and Pupal Wings from the African Butterfly Bicyclus anynana

Immunohistochemistry Staining of Embryos from the African Butterfly Bicyclus anynana

Immunohistochemistry Staining of Wing Discs from the African Butterfly Bicyclus anynana

Extraction and Gas Chromatography Analysis of Adult Pheromones from the African Butterfly Bicyclus anynana

Fresh Weight, Dry Weight, and Fat Content of Adult African Butterflies Bicyclus anynana

Constant Volume Respirometry in the African Butterfly Bicyclus anynana

Hemolymph Extraction from Various Developmental Stages of the African Butterfly Bicyclus anynana

Injection of Chemicals into Pupae of the African Butterfly Bicyclus anynana

Preparation and Immunolabeling of Caenorhabditis elegans

Construction of Adenovirus Vectors with RGD-Modified Fiber for Transductional Targeting

Construction of Fusion Proteins for Transductional Targeting

Construction of Adenovirus Vectors for Transcriptional Targeting

Comet Fluorescence In Situ Hybridization (Comet-FISH): Detection of DNA Damage

Systematic Monitoring of Protein Complex Composition and Abundance by Blue-Native PAGE

Metabolic Footprinting for the Study of Microbial Biodiversity

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