Table of Contents

May 2010; 2010 (5)

Topic Introductions

  • Variance Component Methods for Analysis of Complex Phenotypes

    • Laura Almasy and
    • John Blangero
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.top77

  • Digital Scanned Laser Light Sheet Fluorescence Microscopy

    • Philipp J. Keller and
    • Ernst H. K. Stelzer
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.top78

  • Stabilization of Proteins for Storage

    • Richard J. Simpson
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.top79

Protocols

  • Labeling Cytoskeletal F-Actin with Rhodamine Phalloidin or Fluorescein Phalloidin for Imaging

    • Brad Chazotte
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot4947

  • β-Galactosidase Activity Staining of Frozen Adult Drosophila Retinas

    • Tanya Wolff
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5418

  • Preparation and Analysis of PEGylated Poly-L-Lysine DNA Nanoparticles for Gene Delivery

    • Pamela B. Davis and
    • Tomasz H. Kowalczyk
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5419

  • Luciferase Assay

    • Stephen T. Smale
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5421

  • Chloramphenicol Acetyltransferase Assay

    • Stephen T. Smale
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5422

  • β-Galactosidase Assay

    • Stephen T. Smale
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5423

  • SEC/Reversed-Phase Separation of E. coli Proteins

    • Alex Apffell
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5424

  • Low-Magnification Live Imaging of Xenopus Embryos for Cell and Developmental Biology

    • John B. Wallingford
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5425

  • Preparation of Fixed Xenopus Embryos for Confocal Imaging

    • John B. Wallingford
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5426

  • High-Magnification In Vivo Imaging of Xenopus Embryos for Cell and Developmental Biology

    • Esther K. Kieserman,
    • Chanjae Lee,
    • Ryan S. Gray,
    • Tae Joo Park,
    • and John B. Wallingford
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5427

  • FAST Technique for Agrobacterium-Mediated Transient Gene Expression in Seedlings of Arabidopsis and Other Plant Species

    • Jian-Feng Li and
    • Andreas Nebenführ
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5428

  • Yeast Two-Hybrid System for Studying Protein-Protein Interactions--Stage 1: Construction and Characterization of a Bait Protein

    • Ilya Serebriiskii
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5429

  • Yeast Two-Hybrid System for Studying Protein-Protein Interactions--Stage 2: Transforming and Characterizing the Library

    • Ilya Serebriiskii
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5430

  • Yeast Two-Hybrid System for Studying Protein-Protein Interactions--Stage 3: Screen for Interacting Proteins

    • Ilya Serebriiskii
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5431

  • Yeast Two-Hybrid System for Studying Protein-Protein Interactions--Stage 4: Isolation of Library Plasmid Insert and Second Confirmation of Positive Interactions

    • Ilya Serebriiskii
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5432

  • Visualizing Fetal Mouse Great Blood Vessels by Plastic Casting

    • Chengyu Liu and
    • Jim Martin
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5433

  • DNA Isolation from Rat Tail or Ear

    • Edwin Cuppen
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5434
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May 2010; 2010 (5)
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