Table of Contents

Protocols

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  Neutral Red Staining for Plant Vacuoles

  • Birgit Schwab and
  • Martin Hülskamp

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot4953
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  Treatment of Surfaces with Poly-L-Lysine for Drosophila Cell Cultivation

  • Lucy Cherbas and
  • Peter Cherbas

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5001
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  Generation of Retroviral Particles for the Spleen Necrosis Virus (SNV)-Based Vector System and Their Use in Transduction of Various Cell Types

  • Zahida Parveen,
  • Muhammad Mukhtar,
  • and Roger J. Pomerantz

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5435
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  Production of SV40-Derived Vectors

  • David S. Strayer,
  • Christine Mitchell,
  • Dawn A. Maier,
  • and Carmen N. Nichols

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5436
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  Titering Replication-Defective rSV40 Vectors

  • David S. Strayer,
  • Christine Mitchell,
  • Dawn A. Maier,
  • and Carmen N. Nichols

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5437
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  Purification of RNA by SDS Solubilization and Phenol Extraction

  • Donald C. Rio,
  • Manuel Ares, Jr,
  • Gregory J. Hannon,
  • and Timothy W. Nilsen

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5438
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  Purification of RNA Using TRIzol (TRI Reagent)

  • Donald C. Rio,
  • Manuel Ares, Jr,
  • Gregory J. Hannon,
  • and Timothy W. Nilsen

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5439
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  Ethanol Precipitation of RNA and the Use of Carriers

  • Donald C. Rio,
  • Manuel Ares, Jr,
  • Gregory J. Hannon,
  • and Timothy W. Nilsen

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5440
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  Preparation of Cytoplasmic and Nuclear RNA from Tissue Culture Cells

  • Donald C. Rio,
  • Manuel Ares, Jr,
  • Gregory J. Hannon,
  • and Timothy W. Nilsen

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5441
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  Removal of Ribosomal Subunits (and rRNA) from Cytoplasmic Extracts before Solubilization with SDS and Deproteinization

  • Donald C. Rio,
  • Manuel Ares, Jr,
  • Gregory J. Hannon,
  • and Timothy W. Nilsen

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5442
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  Removal of DNA from RNA

  • Donald C. Rio,
  • Manuel Ares, Jr,
  • Gregory J. Hannon,
  • and Timothy W. Nilsen

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5443
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  Polyacrylamide Gel Electrophoresis of RNA

  • Donald C. Rio,
  • Manuel Ares, Jr,
  • Gregory J. Hannon,
  • and Timothy W. Nilsen

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5444
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  Nondenaturing Agarose Gel Electrophoresis of RNA

  • Donald C. Rio,
  • Manuel Ares, Jr,
  • Gregory J. Hannon,
  • and Timothy W. Nilsen

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5445
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  In Ovo Live Imaging of Avian Embryos

  • Paul M. Kulesa,
  • Caleb M. Bailey,
  • Cameron Cooper,
  • and Scott E. Fraser

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5446
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  Multi-Position Photoactivation and Multi-Time Acquisition for Large-Scale Cell Tracing in Avian Embryos

  • Joseph Steen,
  • Jason A. Morrison,
  • and Paul M. Kulesa

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5447
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  Illumina Sequencing Library Preparation for Highly Multiplexed Target Capture and Sequencing

  • Matthias Meyer and
  • Martin Kircher

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5448
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  Purification of the Mitotic Checkpoint Complex (MCC) and the Anaphase Promoting Complex/Cyclosome (APC/C) from HeLa Cells

  • Valery Sudakin

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5449
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