Table of Contents

Protocols

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  Thawing Embryonic Stem (ES) Cells from a 96-Well Plate

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot4412
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  Transmission Electron Microscopy (TEM) of Plant Tissues

  • Martin Hülskamp,
  • Birgit Schwab,
  • Paul Grini,
  • and Heinz Schwarz

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot4958
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  Transmission Electron Microscopy (TEM) Freeze Substitution of Plant Tissues

  • Detlef Weigel and
  • Jane Glazebrook

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot4959
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  Preparation of Pseudotyped Lentiviral Vectors Resistant to Inactivation by Serum Complement

  • Ghiabe H. Guibinga and
  • Theodore Friedmann

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5420
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  Purification of Epitope-Tagged Transcription Factor IID

  • Michael F. Carey,
  • Craig L. Peterson,
  • and Stephen T. Smale

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5450
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  Purification of Mediator from HeLa Cell Lines Expressing a Flag-Tagged Mediator Subunit

  • Michael F. Carey,
  • Craig L. Peterson,
  • and Stephen T. Smale

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5451
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  In Vivo Imaging of Tumors

  • Edward Brown,
  • Lance L. Munn,
  • Dai Fukumura,
  • and Rakesh K. Jain

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5452
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  Electrophysiological Recordings from the Drosophila Giant Fiber System (GFS)

  • Marcus J. Allen and
  • Tanja A. Godenschwege

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5453
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  Enrichment of Poly(A)⁺ mRNA Using Immobilized Oligo(dT)

  • Donald C. Rio,
  • Manuel Ares, Jr,
  • Gregory J. Hannon,
  • and Timothy W. Nilsen

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5454
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  Homogenization of Mammalian Tissue

  • Richard J. Simpson

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5455
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  Enzymatic Method for Extracting Extracellular DNA in Biofilm Matrix

  • Jianfeng Wu and
  • Chuanwu Xi

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5456
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  Transfection of Cells with DNA Encoding a Visible Fluorescent Protein-Tagged Lipid-Binding Domain

  • Carsten Schultz,
  • Anne B. Neef,
  • Theodorus W. Gadella, Jr,
  • and Joachim Goedhart

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5457
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  Labeling Lipids for Imaging in Fixed Cells

  • Carsten Schultz,
  • Anne B. Neef,
  • Theodorus W. Gadella, Jr,
  • and Joachim Goedhart

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5458
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  Labeling Lipids for Imaging in Live Cells

  • Carsten Schultz,
  • Anne B. Neef,
  • Theodorus W. Gadella, Jr,
  • and Joachim Goedhart

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5459
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  Immunofluorescent Staining of Drosophila Larval Brain Tissue

  • Aric L. Daul,
  • Hideyuki Komori,
  • and Cheng-Yu Lee

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5460
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  EdU (5-Ethynyl-2′-Deoxyuridine) Labeling of Drosophila Mitotic Neuroblasts

  • Aric L. Daul,
  • Hideyuki Komori,
  • and Cheng-Yu Lee

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5461
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  Multicolor Fluorescence RNA In Situ Hybridization of Drosophila Brain Tissue

  • Aric L. Daul,
  • Hideyuki Komori,
  • and Cheng-Yu Lee

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5462
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  Targeting the Zebrafish Optic Tectum Using In Vivo Electroporation

  • Kenric J. Hoegler and
  • John H. Horne

  Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5463
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