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Embedding Embryos for Episcopic Fluorescence Image Capturing (EFIC)

Timothy J. Mohun and
Wolfgang J. Weninger

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot069575

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Embedding Embryos for High-Resolution Episcopic Microscopy (HREM)

Timothy J. Mohun and
Wolfgang J. Weninger

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot069583

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Generation of Volume Data by Episcopic Three-Dimensional Imaging of Embryos

Timothy J. Mohun and
Wolfgang J. Weninger

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot069591

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Photoconversion for Tracking the Dynamics of Cell Movement in Xenopus laevis Embryos

Brook T. Chernet,
Dany S. Adams,
and Michael Levin

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068502

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Embryonic Cell Ablation in Drosophila Using Lasers

Sean T. Sweeney,
Alicia Hidalgo,
J. Steven de Belle,
and Haig Keshishian

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068395

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Imaging Neocortical Neurons through a Chronic Cranial Window

Anthony Holtmaat,
Vincenzo de Paola,
Linda Wilbrecht,
Josh T. Trachtenberg,
Karel Svoboda,
and Carlos Portera-Cailliau

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot069617

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Electroporation and Use of Hepatitis B Virus Envelope L Proteins as Bionanocapsules

Tadanori Yamada,
Joohee Jung,
Masaharu Seno,
Akihiko Kondo,
Masakazu Ueda,
Katsuyuki Tanizawa,
and Shun'ichi Kuroda

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot069534

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A Colony-Forming Assay for Determining the Establishment Efficiency of S/MAR-Containing Nonviral Episomal Expression Vectors

Claudia Hagedorn,
Armin Baiker,
Jan Postberg,
Anja Ehrhardt,
and Hans J. Lipps

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot069500

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Rescue of S/MAR-Containing Nonviral Episomal Expression Vectors

Claudia Hagedorn,
Armin Baiker,
Jan Postberg,
Anja Ehrhardt,
and Hans J. Lipps

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot069518

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Construction of Flow Chambers for Polarized Total Internal Reflection Fluorescence Microscopy (polTIRFM) Motility Assays

John F. Beausang,
Yujie Sun,
Margot E. Quinlan,
Joseph N. Forkey,
and Yale E. Goldman

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot069385

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The Polarized Total Internal Reflection Fluorescence Microscopy (polTIRFM) Processive Motility Assay for Myosin V

John F. Beausang,
Yujie Sun,
Margot E. Quinlan,
Joseph N. Forkey,
and Yale E. Goldman

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot069393

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The Polarized Total Internal Reflection Fluorescence Microscopy (polTIRFM) Twirling Filament Assay

John F. Beausang,
Yujie Sun,
Margot E. Quinlan,
Joseph N. Forkey,
and Yale E. Goldman

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot069401

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The Acquisition and Analysis of Polarized Total Internal Reflection Fluorescence Microscopy (polTIRFM) Data

John F. Beausang,
Yujie Sun,
Margot E. Quinlan,
Joseph N. Forkey,
and Yale E. Goldman

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot069419

TOPIC INTRODUCTIONS

Episcopic Three-Dimensional Imaging of Embryos

Imaging Neuronal Activity with Genetically Encoded Calcium Indicators

Handling S/MAR Vectors

Power Calculations in Genetic Studies

PROTOCOLS

Embedding Embryos for Episcopic Fluorescence Image Capturing (EFIC)

Embedding Embryos for High-Resolution Episcopic Microscopy (HREM)

Generation of Volume Data by Episcopic Three-Dimensional Imaging of Embryos

Photoconversion for Tracking the Dynamics of Cell Movement in Xenopus laevis Embryos

Embryonic Cell Ablation in Drosophila Using Lasers

Imaging Neocortical Neurons through a Chronic Cranial Window

Electroporation and Use of Hepatitis B Virus Envelope L Proteins as Bionanocapsules

A Colony-Forming Assay for Determining the Establishment Efficiency of S/MAR-Containing Nonviral Episomal Expression Vectors

Rescue of S/MAR-Containing Nonviral Episomal Expression Vectors

Construction of Flow Chambers for Polarized Total Internal Reflection Fluorescence Microscopy (polTIRFM) Motility Assays

The Polarized Total Internal Reflection Fluorescence Microscopy (polTIRFM) Processive Motility Assay for Myosin V

The Polarized Total Internal Reflection Fluorescence Microscopy (polTIRFM) Twirling Filament Assay

The Acquisition and Analysis of Polarized Total Internal Reflection Fluorescence Microscopy (polTIRFM) Data

INFORMATION PANEL

Setup for Functional Cell Ablation with Lasers: Coupling of a Laser to a Microscope

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