Table of Contents

August 2015; 2015 (8)

TOPIC INTRODUCTIONS

  • Deep Mutational Scanning: A Highly Parallel Method to Measure the Effects of Mutation on Protein Function

    • Lea M. Starita and
    • Stanley Fields
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.top077503

  • Optogenetics in Freely Moving Mammals: Dopamine and Reward

    • Feng Zhang,
    • Hsing-Chen Tsai,
    • Raag D. Airan,
    • Garret D. Stuber,
    • Antoine R. Adamantidis,
    • Luis de Lecea,
    • Antonello Bonci,
    • and Karl Deisseroth
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.top086330

  • Chimeric Tumor and Organ Transplantation Models

    • Michael Hemann
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.top069872

  • Isolation of Nuclei

    • Arash Nabbi and
    • Karl Riabowol
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.top074583

PROTOCOLS

  • Syngeneic Transplants with Modified Chimeric Hematopoietic Tumors

    • Michael Hemann
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot078014

  • Loss-of-Function Screening in Hematopoietic Malignancies

    • Michael Hemann
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot078022

  • Monitoring Autophagic Flux by Using Lysosomal Inhibitors and Western Blotting of Endogenous MAP1LC3B

    • Suganthi Chittaranjan,
    • Svetlana Bortnik,
    • and Sharon M. Gorski
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot086256

  • Monitoring the Localization of MAP1LC3B by Indirect Immunofluorescence

    • Nicholas T. Ktistakis
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot086264

  • Detection of p62 on Paraffin Sections by Immunohistochemistry

    • Alexander S. Watson and
    • Elizabeth J. Soilleux
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot086280

  • Ca2+ Uncaging in Nerve Terminals: A Three-Point Calibration Procedure

    • Olexiy Kochubey and
    • Ralf Schneggenburger
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot087650

  • Rapid Isolation of Nuclei from Cells In Vitro

    • Arash Nabbi and
    • Karl Riabowol
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot083733

  • Isolation of Pure Nuclei Using a Sucrose Method

    • Arash Nabbi and
    • Karl Riabowol
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot083741

  • Deep Mutational Scanning: Library Construction, Functional Selection, and High-Throughput Sequencing

    • Lea M. Starita and
    • Stanley Fields
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot085225

  • Deep Mutational Scanning: Calculating Enrichment Scores for Protein Variants from DNA Sequencing Output Files

    • Lea M. Starita and
    • Stanley Fields
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot085233

  • Removal of rRNA from Deproteinized, Phenol-Extracted Total RNA by Hybrid Selection

    • Timothy W. Nilsen
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot086132

ERRATA

  • Analysis of Splicing In Vitro Using Extracts of Saccharomyces cerevisiae

    • Manuel Ares Jr.
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.err089888

  • Analysis of Splicing Complexes on Native Gels

    • Manuel Ares Jr.
    Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.err089896
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This Issue

August 2015; 2015 (8)
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  1. TOPIC INTRODUCTIONS
  2. PROTOCOLS
  3. ERRATA

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