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Differentiating Mouse Embryonic Stem Cells into Embryoid Bodies by Hanging-Drop Cultures

Richard Behringer,
Marina Gertsenstein,
Kristina Vintersten Nagy,
and Andras Nagy

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot092429

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Preparation of Intracellular Metabolite Extracts from Liquid Schizosaccharomyces pombe Cultures

Tomáš Pluskal,
Takahiro Nakamura,
and Mitsuhiro Yanagida

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot091553

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Measurement of Metabolome Samples Using Liquid Chromatography–Mass Spectrometry, Data Acquisition, and Processing

Tomáš Pluskal and
Mitsuhiro Yanagida

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot091561

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Analysis of Cytochrome c Release by Immunocytochemistry

Lisa C. Crowley,
Brooke J. Marfell,
Adrian P. Scott,
and Nigel J. Waterhouse

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087338

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Measuring Mitochondrial Transmembrane Potential by TMRE Staining

Lisa C. Crowley,
Melinda E. Christensen,
and Nigel J. Waterhouse

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087361

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Generating Bait Strains for Yeast One-Hybrid Assays

Juan I. Fuxman Bass,
John S. Reece-Hoyes,
and Albertha J.M. Walhout

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot088948

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Performing Yeast One-Hybrid Library Screens

Juan I. Fuxman Bass,
John S. Reece-Hoyes,
and Albertha J.M. Walhout

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot088955

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Colony Lift Colorimetric Assay for β-Galactosidase Activity

Juan I. Fuxman Bass,
John S. Reece-Hoyes,
and Albertha J.M. Walhout

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot088963

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Zymolyase-Treatment and Polymerase Chain Reaction Amplification from Genomic and Plasmid Templates from Yeast

Juan I. Fuxman Bass,
John S. Reece-Hoyes,
and Albertha J.M. Walhout

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot088971

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Precipitation of DNA with Ethanol

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot093377

TOPIC INTRODUCTIONS

Overview of CRISPR–Cas9 Biology

Gene-Centered Yeast One-Hybrid Assays

Metabolomic Analysis of Schizosaccharomyces pombe: Sample Preparation, Detection, and Data Interpretation

In the Midst of Life—Cell Death: What Is It, What Is It Good for, and How to Study It

Dead Cert: Measuring Cell Death

PROTOCOLS

Differentiating Mouse Embryonic Stem Cells into Embryoid Bodies by Hanging-Drop Cultures

Preparation of Intracellular Metabolite Extracts from Liquid Schizosaccharomyces pombe Cultures

Measurement of Metabolome Samples Using Liquid Chromatography–Mass Spectrometry, Data Acquisition, and Processing

Analysis of Cytochrome c Release by Immunocytochemistry

Measuring Mitochondrial Transmembrane Potential by TMRE Staining

Generating Bait Strains for Yeast One-Hybrid Assays

Performing Yeast One-Hybrid Library Screens

Colony Lift Colorimetric Assay for β-Galactosidase Activity

Zymolyase-Treatment and Polymerase Chain Reaction Amplification from Genomic and Plasmid Templates from Yeast

Precipitation of DNA with Ethanol

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