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G-Protein Modulation of Voltage-Gated Ca²⁺ Channels from Isolated Adult Rat Superior Cervical Ganglion Neurons

Van B. Lu and
Stephen R. Ikeda

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot091223

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The Use of Lysosomotropic Dyes to Exclude Lysosomal Membrane Permeabilization

Urška Repnik,
Maruša Hafner Česen,
and Boris Turk

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087106

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Measuring Cysteine Cathepsin Activity to Detect Lysosomal Membrane Permeabilization

Urška Repnik,
Maruša Hafner Česen,
and Boris Turk

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087114

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Coverslip Cleaning and Functionalization for Total Internal Reflection Fluorescence Microscopy

Emily M. Kudalkar,
Yi Deng,
Trisha N. Davis,
and Charles L. Asbury

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot085548

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Preparation of Reactions for Imaging with Total Internal Reflection Fluorescence Microscopy

Emily M. Kudalkar,
Trisha N. Davis,
and Charles L. Asbury

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot085563

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Data Analysis for Total Internal Reflection Fluorescence Microscopy

Charles L. Asbury

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot085571

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Characterizing Cas9 Protospacer-Adjacent Motifs with High-Throughput Sequencing of Library Depletion Experiments

Jonathan L. Braff,
Stephanie J. Yaung,
Kevin M. Esvelt,
and George M. Church

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot090183

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Identifying Products of Recombinase-Mediated Cassette Exchange (RMCE) in Schizosaccharomyces pombe

Johanne M. Murray,
Adam T. Watson,
and Antony M. Carr

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot090944

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Extraction of Chromosomal DNA from Schizosaccharomyces pombe

Johanne M. Murray,
Adam T. Watson,
and Antony M. Carr

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot090985

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Colony Polymerase Chain Reaction with Schizosaccharomyces pombe

Johanne M. Murray,
Adam T. Watson,
and Antony M. Carr

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot090993

TOPIC INTRODUCTIONS

Molecular Genetic Tools and Techniques in Fission Yeast

Classical Genetics with Saccharomyces cerevisiae

Characterization of Cas9–Guide RNA Orthologs

Strategies for Investigating G-Protein Modulation of Voltage-Gated Ca²⁺ Channels

Single-Molecule Total Internal Reflection Fluorescence Microscopy

PROTOCOLS

G-Protein Modulation of Voltage-Gated Ca²⁺ Channels from Isolated Adult Rat Superior Cervical Ganglion Neurons

The Use of Lysosomotropic Dyes to Exclude Lysosomal Membrane Permeabilization

Measuring Cysteine Cathepsin Activity to Detect Lysosomal Membrane Permeabilization

Coverslip Cleaning and Functionalization for Total Internal Reflection Fluorescence Microscopy

Preparation of Reactions for Imaging with Total Internal Reflection Fluorescence Microscopy

Data Analysis for Total Internal Reflection Fluorescence Microscopy

Characterizing Cas9 Protospacer-Adjacent Motifs with High-Throughput Sequencing of Library Depletion Experiments

Identifying Products of Recombinase-Mediated Cassette Exchange (RMCE) in Schizosaccharomyces pombe

Extraction of Chromosomal DNA from Schizosaccharomyces pombe

Colony Polymerase Chain Reaction with Schizosaccharomyces pombe

This Issue

Current Issue

From the cover

Table of Contents

TOPIC INTRODUCTIONS

Molecular Genetic Tools and Techniques in Fission Yeast

Classical Genetics with Saccharomyces cerevisiae

Characterization of Cas9–Guide RNA Orthologs

Strategies for Investigating G-Protein Modulation of Voltage-Gated Ca2+ Channels

Single-Molecule Total Internal Reflection Fluorescence Microscopy

PROTOCOLS

G-Protein Modulation of Voltage-Gated Ca2+ Channels from Isolated Adult Rat Superior Cervical Ganglion Neurons

The Use of Lysosomotropic Dyes to Exclude Lysosomal Membrane Permeabilization

Measuring Cysteine Cathepsin Activity to Detect Lysosomal Membrane Permeabilization

Coverslip Cleaning and Functionalization for Total Internal Reflection Fluorescence Microscopy

Preparation of Reactions for Imaging with Total Internal Reflection Fluorescence Microscopy

Data Analysis for Total Internal Reflection Fluorescence Microscopy

Characterizing Cas9 Protospacer-Adjacent Motifs with High-Throughput Sequencing of Library Depletion Experiments

Identifying Products of Recombinase-Mediated Cassette Exchange (RMCE) in Schizosaccharomyces pombe

Extraction of Chromosomal DNA from Schizosaccharomyces pombe

Colony Polymerase Chain Reaction with Schizosaccharomyces pombe

This Issue

Current Issue

From the cover

Strategies for Investigating G-Protein Modulation of Voltage-Gated Ca²⁺ Channels

G-Protein Modulation of Voltage-Gated Ca²⁺ Channels from Isolated Adult Rat Superior Cervical Ganglion Neurons