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Genetic Methods for Studying Yeast Prions

Reed B. Wickner,
Herman K. Edskes,
Dmitry Kryndushkin,
and Frank P. Shewmaker

Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.prot089029

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Prion Transfection of Yeast

Herman K. Edskes,
Dmitry Kryndushkin,
Frank Shewmaker,
and Reed B. Wickner

Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.prot089037

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Isolation and Analysis of Prion and Amyloid Aggregates from Yeast Cells

Dmitry Kryndushkin,
Natalia Pripuzova,
and Frank P. Shewmaker

Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.prot089045

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Preparation of Slice Cultures from Rodent Hippocampus

Christine E. Gee,
Iris Ohmert,
J. Simon Wiegert,
and Thomas G. Oertner

Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.prot094888

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Viral Vector-Based Transduction of Slice Cultures

J. Simon Wiegert,
Christine E. Gee,
and Thomas G. Oertner

Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.prot094896

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Single-Cell Electroporation of Neurons

J. Simon Wiegert,
Christine E. Gee,
and Thomas G. Oertner

Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.prot094904

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Preparation of Protein Extracts from Schizosaccharomyces pombe Using Trichloroacetic Acid Precipitation

Agnes Grallert and
Iain M. Hagan

Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.prot091579

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Small-Scale Immunoprecipitation from Fission Yeast Cell Extracts

Agnes Grallert and
Iain M. Hagan

Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.prot091587

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Large-Scale Immunoprecipitation from Fission Yeast Cell Extracts

Agnes Grallert and
Iain M. Hagan

Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.prot091595

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Mapping Short Sequence Reads to a Reference Genome

Jui-Hung Hung and
Zhiping Weng

Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.prot093161

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Identifying Regions Enriched in a ChIP-seq Data Set (Peak Finding)

Jui-Hung Hung and
Zhiping Weng

Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.prot093187

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Discovering cis-Regulatory Motifs

Jui-Hung Hung and
Zhiping Weng

Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.prot093203

TOPIC INTRODUCTIONS

Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels

Motif Finding

Prions

PROTOCOLS

Genetic Methods for Studying Yeast Prions

Prion Transfection of Yeast

Isolation and Analysis of Prion and Amyloid Aggregates from Yeast Cells

Preparation of Slice Cultures from Rodent Hippocampus

Viral Vector-Based Transduction of Slice Cultures

Single-Cell Electroporation of Neurons

Preparation of Protein Extracts from Schizosaccharomyces pombe Using Trichloroacetic Acid Precipitation

Small-Scale Immunoprecipitation from Fission Yeast Cell Extracts

Large-Scale Immunoprecipitation from Fission Yeast Cell Extracts

Mapping Short Sequence Reads to a Reference Genome

Identifying Regions Enriched in a ChIP-seq Data Set (Peak Finding)

Discovering cis-Regulatory Motifs

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