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Generating a Three-Dimensional Genome from Xenopus with Hi-C

Ian K. Quigley and
Sven Heinz

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot098343

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Construction of Recombinant Adenovirus Genomes by Direct Cloning

Xiangyang Zhou,
Miguel Sena-Esteves,
and Guangping Gao

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot095521

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Release of the Cloned Recombinant Adenovirus Genome for Rescue and Expansion

Qin Su,
Miguel Sena-Esteves,
and Guangping Gao

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot095539

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Purification of the Recombinant Adenovirus by Cesium Chloride Gradient Centrifugation

Qin Su,
Miguel Sena-Esteves,
and Guangping Gao

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot095547

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Peptide Affinity Purification of Antibodies

Jordan B. Fishman and
Eric A. Berg

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot099176

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Conjugation of Peptides to Thiol-Reactive Gel for Affinity Purification of Antibodies

Jordan B. Fishman and
Eric A. Berg

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot099168

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Assessing Antibody Responses to Pathogens or Model Antigens in Xenopus by Enzyme-Linked Immunosorbent Assay (ELISA)

Francisco De Jesús Andino and
Jacques Robert

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot099234

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Skin Grafting in Xenopus laevis: A Technique for Assessing Development and Immunological Disparity

Yumi Izutsu

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot099788

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Adoptive Transfer of Fluorescently Labeled Immune Cells in Xenopus

Kun Hyoe Rhoo and
Jacques Robert

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot097592

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Observation of Fluorescent Proteins in Living Cells

Richard Behringer,
Marina Gertsenstein,
Kristina Vintersten Nagy,
and Andras Nagy

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot092767

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Observation of Fluorescent Proteins in Fixed Cells

Richard Behringer,
Marina Gertsenstein,
Kristina Vintersten Nagy,
and Andras Nagy

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot092775

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Amplification of cDNA Generated by Reverse Transcription of mRNA: Two-Step Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot095190

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Rapid Amplification of Sequences from the 5′ Ends of mRNAs: 5′-RACE

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot095208

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Rapid Amplification of Sequences from the 3′ Ends of mRNAs: 3′-RACE

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot095216

TOPIC INTRODUCTION

Antibody Purification and Storage

PROTOCOLS

Generating a Three-Dimensional Genome from Xenopus with Hi-C

Construction of Recombinant Adenovirus Genomes by Direct Cloning

Release of the Cloned Recombinant Adenovirus Genome for Rescue and Expansion

Purification of the Recombinant Adenovirus by Cesium Chloride Gradient Centrifugation

Peptide Affinity Purification of Antibodies

Conjugation of Peptides to Thiol-Reactive Gel for Affinity Purification of Antibodies

Assessing Antibody Responses to Pathogens or Model Antigens in Xenopus by Enzyme-Linked Immunosorbent Assay (ELISA)

Skin Grafting in Xenopus laevis: A Technique for Assessing Development and Immunological Disparity

Adoptive Transfer of Fluorescently Labeled Immune Cells in Xenopus

Observation of Fluorescent Proteins in Living Cells

Observation of Fluorescent Proteins in Fixed Cells

Amplification of cDNA Generated by Reverse Transcription of mRNA: Two-Step Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Rapid Amplification of Sequences from the 5′ Ends of mRNAs: 5′-RACE

Rapid Amplification of Sequences from the 3′ Ends of mRNAs: 3′-RACE

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