Table of Contents

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Administering Anesthesia to Mice, Rats, and Hamsters

Edward A. Greenfield

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot100198

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Routes of Antigen Injection in Mice and Rats

Edward A. Greenfield

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot100230

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Special Considerations for Making Explants and Transplants with Xenopus tropicalis

Marilyn Fisher and
Robert M. Grainger

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot097428

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INTACT Proteomics in Xenopus

Lauren Wasson,
Nirav M. Amin,
and Frank L. Conlon

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot098384

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Mass Spectrometry-Based Absolute Quantification of Single Xenopus Embryo Proteomes

Rik G.H. Lindeboom,
Arne H. Smits,
Matteo Perino,
Gert Jan C. Veenstra,
and Michiel Vermeulen

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot098376

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Assessing Ubiquitylation of Individual Proteins Using Xenopus Extract Systems

Gary S. McDowell and
Anna Philpott

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot104513

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Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems

Gary S. McDowell and
Anna Philpott

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot103481

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An RNA-Seq Protocol for Differential Expression Analysis

Nick D.L. Owens,
Elena De Domenico,
and Michael J. Gilchrist

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot098368

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Random Scanning Mutagenesis

Matteo Forloni,
Alex Y. Liu,
and Narendra Wajapeyee

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot097808

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Megaprimer Polymerase Chain Reaction (PCR)-Based Mutagenesis

Matteo Forloni,
Alex Y. Liu,
and Narendra Wajapeyee

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot097824

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Screening Colonies by Polymerase Chain Reaction (PCR)

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot095224

TOPIC INTRODUCTIONS

The Use of Cell-Free Xenopus Extracts to Investigate Cytoplasmic Events

Polymerase Chain Reaction

PROTOCOLS

Administering Anesthesia to Mice, Rats, and Hamsters

Routes of Antigen Injection in Mice and Rats

Special Considerations for Making Explants and Transplants with Xenopus tropicalis

INTACT Proteomics in Xenopus

Mass Spectrometry-Based Absolute Quantification of Single Xenopus Embryo Proteomes

Assessing Ubiquitylation of Individual Proteins Using Xenopus Extract Systems

Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems

An RNA-Seq Protocol for Differential Expression Analysis

Random Scanning Mutagenesis

Megaprimer Polymerase Chain Reaction (PCR)-Based Mutagenesis

Screening Colonies by Polymerase Chain Reaction (PCR)

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