Recipe

PEM (2X)

7.56 g PIPES (dipotassium salt)

152 mg EGTA

2 ml MgSO4 (0.1 M)

Add H2O to make 100 ml. Adjust pH to 7.0. Filter-sterilize and store at 4°C for up to 1 year.

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  1. doi:10.1101/pdb.rec10920 Cold Spring Harb Protoc 2007: pdb.rec10920-
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