IB buffer
| Reagent | Quantity | |
|---|---|---|
| 1 M Tris-Cl (pH 9.5) | 2 mL | |
| 1 M MgCl2 | 1 mL | |
| 5 M NaCl | 400 μL | |
| H2O | 18 mL | |
| 20% Tween 20 | 100 μL | |
| Keep autoclaved stocks of Tris, MgCl2 and NaCl at room temperature. Add the reagents in order. Mix after addition of each reagent. | ||
| Reagent | Quantity | |
|---|---|---|
| 1 M Tris-Cl (pH 9.5) | 2 mL | |
| 1 M MgCl2 | 1 mL | |
| 5 M NaCl | 400 μL | |
| H2O | 18 mL | |
| 20% Tween 20 | 100 μL | |
| Keep autoclaved stocks of Tris, MgCl2 and NaCl at room temperature. Add the reagents in order. Mix after addition of each reagent. | ||