Recipe

IB buffer

Reagent Quantity
1 M Tris-Cl (pH 9.5) 2 mL
1 M MgCl2 1 mL
5 M NaCl 400 μL
H2O 18 mL
20% Tween 20 100 μL
Keep autoclaved stocks of Tris, MgCl2 and NaCl at room temperature. Add the reagents in order. Mix after addition of each reagent.
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  1. doi:10.1101/pdb.rec11887 Cold Spring Harb Protoc 2009: pdb.rec11887-
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