NB buffer
| Reagent | Quantity |
|---|---|
| 1 M Tris-Cl (pH 9.5) | 20 mL |
| 1 M MgCl2 | 1 mL |
| 5 M NaCl | 400 μL |
| 20% Tween 20 | 100 μL |
| Keep autoclaved stocks of Tris-Cl, MgCl2 and NaCl at room temperature. Add the reagents in order, mixing after each addition. Prepare fresh NB buffer before each use. | |
