Recipe

ESP solution

EDTA (0.5 M, pH 8.0)

Sarkosyl (1%, w/v)

Autoclave. Immediately before use, add:

Proteinase K (1.4 U/mg; 1 mg/mL)

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  1. doi:10.1101/pdb.rec11917 Cold Spring Harb Protoc 2009: pdb.rec11917-
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