Table

Table 2. Bait characterization: Expected results

Anticipated results
Assay Plasmids to transform X-gal plates Growth Leu-
Reporter LexA-fusion Glu Gal/Raf Gal/Raf
Activation:
Test pMW112 pBait (White/light blue) (White/light blue) (No)
Negative control pMW112 pEG202-Ras White White No
Weak positive control pMW112 pEG202-hsRPB7 Light blue Bluish No
Strong positive control pMW112 pSH17-4 Dark blue Blue Yes
Repression:
Test pJK101 pBait (White) (Lighter blue)
Negative control pJK101 pGKS3a White Blue No
Positive control pJK101 pEG202-Ras White Lighter blue
Test by Western blot
Expression:
Test Use clones from activation assay pBait (Single band)
Positive control 1 pEG202-Ras Single band ~45 kD
Positive control 2 pSH17-4 Single band ~35 kD
apGKS3 can be substituted for any other yeast HIS3 plasmid not expressing LexA.
(Adapted from Table 18-7, Sambrook and Russell 2001.)
| Table of Contents

This Article

  1. doi:10.1101/pdb.tab295429 Cold Spring Harb Protoc 2010: pdb.tab295429-
  1. » Full Text

Article Category

  1. Table

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. January 2026, 2026 (1)
  1. Current Issue