Primary DC medium

Iscove’s modified Dulbecco’s medium (IMDM)

β-mercaptoethanol (50 μM)

Fetal bovine serum, heat-inactivated (10%)

L-Glutamine (2 mM)

Granulocyte macrophage colony-stimulating factor (GM-CSF; 20-30 ng/mL)

Interleukin-4 (IL-4; 100-400 IU/mL)

Penicillin (100 IU/mL)

Streptomycin (100 μg/mL)

Sterilize. Medium can be stored for ~3 mo.