Recipe

Regeneration Medium

Reagent Quantity (for 1 L) Final concentration
MgCl2·6H2O (MW = 203.3) 30.5 g 150 mm
KCl (MW = 74.55) 7.5 g 100 mm
NaHCO3 (MW = 84.01) 0.34 g 4 mm
KNO3 (MW = 101.11) 0.202 g 2 mm
NaH2PO4 (MW = 119.98) 0.012 g 100 µm

  • Adjust the volume with natural seawater.

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    1. doi:10.1101/pdb.rec068734 Cold Spring Harb Protoc 2012: pdb.rec068734- © 2012 Cold Spring Harbor Laboratory Press
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