Sorbitol Buffer for Yeast Extracts
| Reagent | Quantity (for 100 mL) | Final concentration |
|---|---|---|
| Sorbitol | 18.2 g | 1 m |
| Tris-HCl (1 m, pH 7.8) | 5 mL | 50 mm |
| MgCl2 (1 m) | 1 mL | 10 mm |
| H2O | to 100 mL | |
| Sterile filter and store at 4°C. | ||
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