ICRAC Ca2+-Containing Bath Solution
| NaCl | 120 mm |
| Tetraethylammonium chloride (TEA-Cl) | 10 mm |
| CaCl2* | 10 mm |
| MgCl2 | 2 mm |
| Glucose | 30 mm |
| HEPES | 10 mm |
| Adjust pH to 7.2 with NaOH (not below 7.2 because CRAC channels are blocked by low pH, a pH of up to 7.4 can also be used). Adjust osmolarity to around 320 mOsm. Sterile-filter before storage. | |
| *The amount of CaCl2 is determined by the cells’ tolerance and the current size and can vary between 0.5 and 20 mm CaCl2. | |
- © 2014 Cold Spring Harbor Laboratory Press
