Urea Loading Dye
| Reagent | Quantity (for 10 mL) | Final concentration |
|---|---|---|
| Urea | 4.8 g | 8 m |
| Tris–HCl (1 m, pH 8.0) | 200 µL | 20 mm |
| EDTA (0.25 m) | 40 µL | 1 mm |
| Bromphenol blue/xylene cyanol (1%, w/v) | 0.5 mL | 0.05% (w/v) |
| ddH2O | to 10 mL |
- © 2014 Cold Spring Harbor Laboratory Press
