Solution A for Organotypic Culture
| Reagent | Volume |
|---|---|
| Eagle's minimal essential medium (EMEM; 10×, Lonza 12-684F) | 690 µL |
| Fetal bovine serum | 700 µL |
| l-Glutamine (200 mm; Cellgro 25-005CI) | 60 µL |
| Sodium bicarbonate (NaHCO3; Lonza 17-613E) | 140 µL |
| Bovine collagen type I (Organogenesis 200-055) | 5.6 mL |
| Combine the reagents in the order listed. The final volume is sufficient for six inserts. | |
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