External Recording Solution for Ih Recording
| Reagents | Final concentration |
|---|---|
| NaCl | 125 mm |
| KCl | 2.5 mm |
| NaH2PO4 | 1.25 mm |
| NaHCO3 | 25 mm |
| CaCl2 | 2 mm |
| MgCl2 | 2 mm |
| Glucose | 10 mm |
| Tetrodotoxin | 0.001 mm |
| CdCl2 | 0.1 mm |
| Tetraethylammonium chloride (TEA-Cl) | 10 mm |
| 4-aminopyridine (4-AP) | 1 mm |
| BaCl2 | 1 mm |
| Bubble with 95% O2/5% CO2. (After bubbling, the solution will have a pH of 7.2; osmolarity will be ~320 mOsm. Ideally, this solution should be made up fresh on the day of the experiment and kept bubbling throughout the experiment.) | |
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