Internal Pipette Solution for Ih Recording in Cell-Attached Mode
| Reagents | Final concentration |
|---|---|
| KCl | 120 mm |
| Tetraethylammonium chloride (TEA-Cl) | 20 mm |
| 4-aminopyridine (4-AP) | 5 mm |
| BaCl2 | 1 mm |
| HEPES | 10 mm |
| MgCl2 | 1 mm |
| CaCl2 | 2 mm |
| Tetrodotoxin | 0.001 mm |
| NiCl2 | 0.1 mm |
| Adjust the pH of the solution to 7.3. (The osmolarity will be 300 mOsm.) Store at 4°C for up to 1 mo. | |
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