Internal Pipette Solution for Ih Recording in Whole-Cell Voltage-Clamp Mode or Outside-Out Patch Mode
| Reagents | Final concentration |
|---|---|
| KMeSO4 | 120 mm |
| KCl | 20 mm |
| 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) | 10 mm |
| MgCl2 | 2 mm |
| Ethylene glycol-bis(2-aminoethylether)-N,N,N′, N′-tetraacetic acid (EGTA) | 0.2 mm |
| Na2ATP | 4 mm |
| Tris–GTP | 0.3 mm |
| Tris–phosphocreatine | 14 mm |
| Adjust the pH of the solution to 7.3. (The osmolarity will be 300 mOsm.) Aliquot and store at −20°C for up to 3 mo. Keep the solution on ice during use. | |
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