Recipe

Final Medium

Reagent Final concentration
BDNF (R&D Systems 248-BD-025/CF)  20 ng/mL
Bovine serum albumin (Sigma-Aldrich) 0.5 mg/mL
EGF (R&D Systems 2028-EG-200)  20 ng/mL
FGF (R&D Systems 3139-FB-025/CF)  20 ng/mL
Prepare fresh in L-15 Medium (Sigma-Aldrich L4386).
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  1. doi:10.1101/pdb.rec104737 Cold Spring Harb Protoc 2018: pdb.rec104737- © 2018 Cold Spring Harbor Laboratory Press

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