Recipe

Frog Water

Reagent Amount (200 L) Final concentration
Na2HPO4 (0.4 m)   1 L   2 mm
Instant Ocean Sea Salt solution   1 L 0.5 g/L
Reverse osmosis (RO) water 198 L    -
Prepare frog water in a 200-L carboy. Frog water can be stored long-term at room temperature. To prevent the growth of green algae, periodically scrub the carboy with a brush without detergent, and rinse with water.
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  1. doi:10.1101/pdb.rec104786 Cold Spring Harb Protoc 2018: pdb.rec104786- © 2018 Cold Spring Harbor Laboratory Press

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