Recipe

TT Buffer

Reagent Amount to add (for 50 mL) Final concentration
Tris-HCl (1 m, pH 8.0)  500 µL 10 mm
Tween 20 (10%)  250 µL 0.05%
ddH2O (sterile) 49.25 mL
Store for up to 6 mo at 4°C.
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  1. doi:10.1101/pdb.rec106450 Cold Spring Harb Protoc 2019: pdb.rec106450- © 2019 Cold Spring Harbor Laboratory Press
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