Recipe

Coomassie Brilliant Blue R-250 Stain Solution

Reagent Quantity (for 100 mL) Final concentration
Coomassie Brilliant Blue R-250  0.05 g   0.05%
Methanol  50 mL 50% (v/v)
Glacial acetic acid  10 mL 10% (v/v)
H2O to 100 mL
Dissolve the Coomassie Brilliant Blue R-250 dye, and then filter through a Whatman No. 1 filter to remove any particulate matter. Can be stored at room temperature.
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  1. doi:10.1101/pdb.rec105080 Cold Spring Harb Protoc 2020: pdb.rec105080- © 2020 Cold Spring Harbor Laboratory Press

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