Internal Recording Solution for Whole-Cell Electrophysiological Recordings
| Reagent | Final concentration |
|---|---|
| K-gluconate | 100 mm |
| KCl | 8 mm |
| NaCl | 5 mm |
| MgCl2 | 1.5 mm |
| HEPES | 20 mm |
| EGTA | 10 mm |
| ATP | 2 mm |
| GTP | 0.3 mm |
| Prepare the solution with deionized H2O. Adjust the pH to 7.2 using 1 n KOH, and adjust osmolarity to 255 mOsm using sucrose. Store at −80°C. | |
