Recipe

Mild Iodine Solution

Dissolve two Ringer's tablets (Millipore 96724) in 1 L of MilliQ-purified H2O or reverse-osmosis-treated H2O. Dilute a povidone–iodine solution (10% [w/v], 1% [w/v] available iodine; RICCA 3955-16) in the Ringer's solution at the final concentration of 12–25 ppm iodine. Confirm the iodine concentration with an iodine test strip (Fisher Scientific S72405). Protect the solution from light and leave at room temperature during use. Prepare fresh before use.

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  1. doi:10.1101/pdb.rec108126 Cold Spring Harb Protoc 2023: pdb.rec108126- © 2023 Cold Spring Harbor Laboratory Press

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