Phosphate-Buffered Saline with Tween and BSA (PBSTB)
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1. Combine the following salt components in distilled H2O.
| Reagent | Final concentration |
|---|---|
| NaCl | 150 mm |
| Na2HPO4 | 8 mm |
| NaH2PO4 | 2 mm |
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2. Adjust salt components and distilled H2O to reach pH 7.4 by using NaOH or HCl for a final volume of 1 L.
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3. Autoclave the solution at 15 PSI for 20 min at 121°C on a liquid cycle. Verify the pH again.
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4. Add Tween 20 and dissolve BSA to final concentrations of 0.1% (w/v) and 5% (w/v), respectively. Adjust the volume to 1 L and sterilize with a 0.2-µm filter. Prepare fresh before use. Keep at 4°C.
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Different concentrations of BSA (1%–5%, w/v) are used.
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5. When required, dilute PBSTB (5% BSA) with PBST to prepare solutions with lower concentrations of BSA.
